Fig. 3. Anti-plasmid activity of the LdCsm system.
a Schematic of the test plasmids pBad-G, pBad-CTR, and pBad-NTR. Gene expression from these plasmids is under the control of a L-arabinose-inducible Bad promoter. Therefore, mRNAs carrying CTR or NTR are expressed from the corresponding plasmids (pBad-CTR or pBad-NTR) in the presence of L-arabinose, but their expression is completely repressed in glucose media. b Schematic of the interference plasmid assay for determination of the LdCsm anti-plasmid activity. c Transformation efficiency data of the three different plasmids obtained from different growth media.