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. 2020 May 11;11(5):351. doi: 10.1038/s41419-020-2561-6

Fig. 2. siRNA was selected for silencing SETDB1 or lentivirus overexpressing SETDB1 in CRC cells.

Fig. 2

a At 24 h post transfection, fluorescence photographs of CRC cells transfected with FAM-labeled siRNA using lipo2000. SW480: Scale bar, 50 µm; HCT116: Scale bar, 200 µm. b qRT-PCR analysis of SETDB1 expression following the treatment of SW480 and HCT116 cells with lenti-SETDB1 or the lenti-NC. c, d SETDB1 mRNA and protein expression in SW480 and HCT116 after transfected with siSETDB1-1, 2, 3 or the negative control. e Gray analysis of WB results by image J software. Error bars indicate mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ns means no significance.