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. 2020 May 11;11:2330. doi: 10.1038/s41467-020-16231-7

Fig. 7. Cell binding and T cell redirected killing properties of the TCR/CD3 BsAbs.

Fig. 7

a Flow cytometry cell binding titrations of the BsAb molecules with HLA-A2+ Saos-2 tumor cells with or without pulsing of the NY-ESO-1 peptide SLLMWITQC (left) and with CD3+ Jurkat cells (right). Each data point is the Geometric mean of the fluorescence of the cells labeled with the CD3 mAb or TCR/CD3 BsAb and secondary antibody. b T cell redirected killing of HLA-A2+ Saos-2 (left) and 624.38 (right) tumor cells facilitated by TCR/CD3 BsAbs with and without pulsing with exogenous NY-ESO-1 peptide. c TCR/CD3 tandem BsAb-mediated T cell redirected killing of A375 HLA-A2+/NY-ESO-1+ melanoma cells (no addition of exogenous peptide). Panels to the right and left use T cells expanded from different donors. For plots in sections (b) and (c), the data points are the mean of three replicates and the error bars are the standard deviation. Source data are available in the Source Data file.