Fig. 4. CC3 is preferentially upregulated in cochlear cultures obtained from Cx43G60S/+ mutant mice.
a Representative confocal images of the apical, middle, and basal regions of organotypic cochlear cultures from WT and Cx43G60S/+ mice labeled for CC3 after being untreated or treated with 20 μM cisplatin. CC3 is denoted in red, phalloidin in green, and nuclei in blue. b CC3-positive hair cells were quantified as hair cells labeled positive for CC3 and phalloidin. N values; WT untreated = 5, WT cisplatin = 5–6, Cx43G60S/+ untreated = 4, Cx43G60S/+ cisplatin = 3. Means represent mean ± standard error. Scale bars = 20 μm. One-way ANOVA’s were performed for each cochlear region and subsequently a post hoc Tukey’s test was performed. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.