Fig. 7. Carbenoxolone blocks calcein dye transfer in cochlear supporting cells.

a Representative images of prebleach, bleached, and dye recovery after photobleach of calcein fluorescence in untreated or CBX-treated cochlear culture regions. Dashed areas represent photobleached regions. The photobleached region of inner sulcus cells was 288.5 µm². b Percentage of fluorescence recovery compared with baseline in the inner and outer sulcus regions of untreated or 100 μM CBX-treated cochlear cultures. c Area under the curve for both inner and outer sulcus regions revealed that CBX was an effective inhibitor of dye transfer. N = 3, n = 6 in each group and for each region. CBX carbenoxolone treated. Scale bars = 10 μm. Linear regression analysis of slopes performed for each region and area, ****p < 0.0001.