FIGURE 3.

Small RNA (sRNA) profiling of Phakopsora pachyrhizi target genes in soybean leaf tissue that had been rub‐inoculated with modified BPMV containing either EV or HIGS constructs. Number of reads that mapped to various positions of the target genes (a), (c), and (e) and their corresponding read length distributions (b), (d), and (f) of gene‐specific sRNAs identified through RNA‐Seq analysis of the sRNA libraries prepared from leaf samples treated with BPMV:ATC (a) and (b), BPMV:GCS_H (c) and (d) or BPMV:RP_S16 (e) and (f). ATC, acetyl‐CoA acyltransferase; GCS_H, glycine cleavage system H protein; RP_S16, 40S ribosomal protein S16