. 2020 Mar 20;21(6):794–807. doi: 10.1111/mpp.12931

TABLE 3.

List of primers used for real‐time quantitative reverse transcription PCR and in vitro double stranded RNA synthesis

Gene product	Primer name and sequence (5ʹ–3ʹ)		Amplicon size (bp)
Glycine max Ubiquitin‐3	U3F	GTGTAATGTTGGATGTGTTCCC	107
Glycine max Ubiquitin‐3	U3R	ACACAATTGAGTTCAACACAAACCG	107
P. pachyrhizi α‐tubulin	TUBF	CCAAGGCTTCTTCGTGTTTCA	67
P. pachyrhizi α‐tubulin	TUBR	CAAGAGAAGAGCGCCAAACC	67
TaqMan probe	5ʹ FAM–3ʹ Blackhole1	TCGTTTGGAGGCGGATCGGTTCA
	CytB_1_F	TCAAGACGCATCCAATTCTAGGTC	89
	CytB_1_R	GTGTTACACCCGTGATAATCTGAATGAT	89
	PPATC_F	GAGGAGCTGCAAATGGGTGA	115
	PPATC_R	GAATGGGGATGGCAGCATCA	115
	PP_Un_3_F	CGGATCTCAGGATCTCACACG	91
	PP_Un_3_R	GCCAGCTCTCCAGGCTTAAA	91
	PP_GCS_H_F	AGCCGTCGAGAGTGTCAAAG	110
	PP_GCS_H_R	AGATTGGCCTGGTCGCTTAG	110
	PP_RP_S16_F	AAATTTGGAGGAAAGGGAGCACG	89
	PP_RP_S16_R	ACAGCAGGAAATAAAAACCCAAACC	89
P. pachyrhizi ATC dsRNA	ATC_T7_F	GCGTAATACGATCCATCATAGGGAGAGTTTCCATCGATGATGGCATTC	741
P. pachyrhizi ATC dsRNA	ATC_T7_R	GCGTAATACGATCCATCATAGGGAGA CTAGCAAGCCAAACCATAAGC	741
P. pachyrhizi GCS_H dsRNA	GCS_H_T7_F	GCGTAATACGATCCATCATAGGGAGAAAACCATCGTAATCTGCCCG	420
P. pachyrhizi GCS_H dsRNA	GCS_H_T7_R	GCGTAATACGATCCATCATAGGGAGATCCTCTCCTTCACAATGAGCT	420
P. pachyrhizi RP_S16 dsRNA	RP_S16_T7_F	GCGTAATACGATCCATCATAGGGAGACCAGCCTCACAAAACGTTCA	500
P. pachyrhizi RP_S16 dsRNA	RP_S16_T7_R	GCGTAATACGATCCATCATAGGGAGAAACCCAAACCTCCATCAACG	500