Figure 3.

Identification and multiple sequence analysis of the miR159‐targeted LrGAMYB gene. (a) Amino acid alignment of the LrGAMYB protein with MYB homologues in Arabidopsis thaliana, Oryza sativa Japonica group, and Zea mays. (b) The phylogenetic tree of MYBs of Lilium regale and 37 species was constructed using the maximum‐likelihood method. (c) The RNA ligase‐mediated rapid amplification of cDNA ends (RLM‐RACE) products for the predicted LrGAMYB gene amplified and positions of the cleavage. Mapping of lre‐miR159a‐guided cleavage sites in LrGAMYB mRNA by RLM‐RACE. Vertical arrow indicates the 5′ position of the cleaved mRNA fragment, and the number indicates the number of independent clones analysed in the different tissues. (d) Quantitative reverse transcription PCR analysis of LrGAMYB in L. regale after Botrytis elliptica inoculation