Table 1.
Oligonucleotide primers used in the expression analysis of chloroplast Fe homeostasis-related transporters PIC1, NiCo and MAR1
| Gene | Genbank accession | Arabidopsis ortholog | Primers | Primer sequences | Melting Tm (°C) | Efficiency | Product size (base pairs) |
|---|---|---|---|---|---|---|---|
| BnPIC1 | Bra036409 | At2g15290 | fw | 5′- TGCGGTCACTACTCTTGC -3′ | 59 | 2.05 | 161 |
| rev | 5′- GATGGTGGCTCTCCTCTTC -3′ | ||||||
| BnNiCo | Bra037287 | At2g16800 | fw | 5′- CTTCCGCCACAATCCTTC -3′ | 59 | 1.88 | 122 |
| rev | 5′- CATAACTCCGCACGATCC -3′ | ||||||
| BnMAR1 | Bra020559 | At5g26820 | fw | 5′-GGCTCTTCTCAGACAATCTCC-3′ | 59 | 1.90 | 98 |
| rev | 5′-TGCGAACTCCAGACAAACC-3′ | ||||||
| 18S rRNA | KT225373 | At2g16590 | fw | 5′- GCATTCGTATTTCATAGTCAGAGGTG-3′ | 61 | 1.87 | 192 |
| rev | 5′- CGGAGTCCTAAAAGCAACATCC-3′ | ||||||
| ß-tubulin | XM_009125342.1 | At4g20890 | fw | 5′- TCSATCCAGGARATGTTCAGG-3′ | 59 | 1.91 | 148 |
| rev | 5′- ACTCTGCAACAAGATCATTCATG-3′ |