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. 2020 Mar 12;43(3):431–444. doi: 10.1007/s13402-020-00497-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — JQ1 efficiently decreases the expression of CSC markers in MDA-MB-231 (a) and BT549 (b) spheroids. c. Effect of JQ1 on the classical stemness markers SOX2 and KLF4. d. Relative mRNA levels of CD44, CD24 and ALDH1A in spheroids exposed to JQ1 in MDA-MB-231 and BT549 spheroids, respectively. The results shown represent the average of 3 independent experiments performed in triplicate. Student t-test: * p < 0,05, ** p < 0,01 and *** p < 0,001