Figure 8.
Cytotoxicity of MM pt-derived CS1-BATs. Cryopreserved PBMC from 2 MM pts from WSU and 2 pts from UVA were used to prepare ATC by activation with OKT3 and expansion in IL2-supplemented media for up to 14 days. ATC were armed with CS1Bi at 50 ng/106 ATC and co-cultured with OPM2 (n = 4) at 2.8–3.6 E:T and ARH77 (n = 3) cells at 3.5–4.0 E:T for 16 h. CS1-BATs from a normal donor VA05 (3.2–3.8 E:T) were included as a positive control for each cell line. The level of cytotoxicity of the pt samples was comparable to that of the normal donor performed in parallel (*P < 0.05).