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. 2020 May 5;10:127. doi: 10.3389/fcimb.2020.00127

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Copyright © 2020 Sheikh, Kumar, Sharma, Bhagyawant and Thavaselvam.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of the sensitivity of colorimetric LAMP, conventional PCR and qPCR. (A) Results of colorimetric LAMP analysis, (B) Agarose gel electrophoresis of colorimetric LAMP products, (C) Agarose gel electrophoresis of conventional PCR products (D) Results of qPCR Analysis, (E) Standard curve and amplification efficiency, (F) Melting curve analysis. Lane, M: DNA marker; Lanes 1-9: Reaction results from a 10-fold serial dilution of plasmid containing the transposase gene from 3 × 10⁷ to 3 × 10⁻¹ copies per reaction; Lane 10: Negative control (nuclease free water).