Table 2.
Relevant splice variants of SLCO1B1 in 97 pediatric liver samples for which the presence in the samples is confirmed by RT‐PCR
| Splice variant | Abundance of all novel isoforms (%) | Abundance compared with reference isoform (%) | Found in number of samples | Number of exons | Length (nt) | ORF (n AA) | Overlapping number of AA with locus: | Number of TM helices | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ORF SLCO1B1 (% of reference SLCO1B1) | Intron SLCO1B1 | ORF SLCO1B7 | In between SLCO1B1 and SLCO1A2 a , b | SLCO‐1A2 a , b | ||||||||
| 46 a , b | 26.55 | 16.48 | 63 | 10 | 4,638 | 284 | 274 (40%) | 10 | – | – | – | 6 |
| 50 a , b | 14.26 | 8.85 | 93 | 17 | 34,388 | 453 | 444 (65%) | 9 | – | – | – | 10 |
| 34 a , b | 9.24 | 5.73 | 46 | 18 | 4,156 | 625 | 622 (90%) | – | – | 3 | – | 11 |
| 24 a , b | 0.32 | 0.20 | 77 | 25 | 3,151 | 659 | 622 (90%) | – | 0 | – | 37 | 11 |
| 26 a , b | 1.80 | 1.12 | 81 | 24 | 13,158 | 691 | 691 (100%) | – | 0 | – | – | 12 |
| 28 a , b | 1.21 | 0.75 | 96 | 20 | 14,577 | 691 | 691 (100%) | – | 0 | – | – | 12 |
| 210 | – | – | – | – | 210 | 0 | ||||||
| 881 | – | – | – | – | 881 | 1 | ||||||
| 30 a , b | 0.34 | 0.21 | 54 | 20 | 5,684 | 484 | 453 (66%) | – | 0 | – | 31 | 8 |
| 38 a , b | 0.52 | 0.33 | 95 | 15 | 22,310 | 453 | 444 (65%) | 9 | – | – | – | 10 |
| 44 a , b | 7.86 | 4.88 | 65 | 3 | 7,364 | 98 | 98 (14%) | – | – | – | – | 2 |
| 51 a , b | 0.73 | 0.46 | 76 | 17 | 15,407 | 522 | 522 (75%) | – | – | – | – | 8 |
AA, amino acid; nt, nucleotides; ORF, open reading frame; RT‐PCR, real‐time polymerase chain reaction; TM, transmembrane.
a
SLCO1A2 is on the reverse strand. bAbundant splice variant. cHybrid SLCO1B1 and ‐1B7. dAge‐related changes in expression.