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. 2020 Mar 12;9(6):686–696. doi: 10.1002/sctm.19-0261

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© 2020 The Authors. stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Histological analysis of the transplanted HA‐iPSC1‐EC/F8 in NSG mice. Neonatal NSG mice were transplanted with HA‐iPSC1‐EC/F8 at their left hind limb. Sixteen weeks after the transplantation, a limb tissue with bioluminescence signal was immunostained with antibodies against GFP, human CD31, and human FVIII. Fluorescence images for GFP (green), human CD31 (red), and human FVIII (white) and the merge image of all three were shown. Nuclei were shown in blue (DAPI stain). Scale bar = 100 μm. ECs, endothelial cells; FVIII, factor VIII; GFP, green fluorescent protein; HA, hemophilia A; iPSC, induced pluripotent stem cell; NSG, NOD/SCID/IL2Rγ^−/−