Figure 5.

Evaluation of the effect of HA‐iPSC‐ECs/F8 on blood loss in B6F8 HA mice. Neonatal B6F8 HA mice were transplanted with HA‐iPSC1‐EC/F8 and analyzed 1 week after the transplantation. A, A representative bioluminescent image of the mice. B, Blood loss in non‐transplanted B6F8 mice or HA‐iPSC1‐EC/F8‐transplanted B6F8 mice and wild‐type C57BL6 (WT) mice in a tail‐clip assay. (n = 4‐5) *P < .05. C, The F8 mRNA in non‐transplanted and HA‐iPSC1‐EC/F8 transplanted HA mice as detected by qRT‐PCR and agarose gel electrophoresis. ECs, endothelial cells; HA, hemophilia A; iPSC, induced pluripotent stem cell; qRT‐PCR, quantitative reverse transcription polymerase chain reaction