Figure 6.

Evaluation of HA‐iPSC‐ECs/F8 in immune deficient F8RG mouse model. Adult F8RG mice were subcutaneously transplanted with HA‐iPSC1‐EC/F8 at their hind limbs and were analyzed 2 weeks after the transplantation. A, A transplanted cell‐Matrigel plug at the transplantation site. B, Immunostaining of a transplanted cell‐Matrigel plug retrieved from the mice for GFP (green), human FVIII (red), and DAPI (blue). Scale bar = 100 μm. C, Plasma FVIII levels in non‐transplanted and transplanted F8RG mice were assayed by FVIII antigen ELISA (n = 7). *P = .015. ECs, endothelial cells; ELISA, enzyme linked immunosorbent assay; FVIII, factor VIII; GFP, green fluorescent protein; HA, hemophilia A; iPSC, induced pluripotent stem cell