Figure 1.

TPO enhances homing of HSPCs to the BM. A, Schematic representation of the homing experiments. Lethally irradiated CD45.2⁺ mice were transplanted with CD45.1⁺ BM MNCs and injected with single doses of TPO at 50 μg/kg or PBS as a control. BMs were harvested for homing assays 20 hours later. B,C, The percentage and homing efficiency of donor CD45.1⁺ HSPCs (LS, LK, and LSK cells) in the recipient BM (n = 3 each). D,E, The homing efficiency of CFU as calculated by comparing the homed CFU numbers with the initially injected CFU numbers. *P < .05, **P < .01, ***P < .001. TPO treatment group vs control group. BFU‐E, burst‐forming unit‐erythrocyte; CFU‐G/GM/M, colony‐forming unit‐granulocyte/granulocyte and macrophage/macrophage; CFU‐GEMM, colony‐forming unit‐granulocyte, erythrocyte, monocyte/macrophage, and megakaryocyte (n = 3 each). BM, bone marrow; HSPC, hematopoietic stem and progenitor cell; MNC, mononuclear cell; PBS, phosphate‐buffered saline; TPO, thrombopoietin