FIGURE 5.

Loss of lipin-1 does not alter systemic immune responses. (A) Splenocytes and (B) blood cells were stained with a panel of antibodies to quantify monocyte/macrophage and PMN populations. Myeloid populations were defined as CD45⁺, CD3^–CD19^–CD11b⁺. PMNs were CD11b⁺ Ly6g⁺ and monocytes were CD11b⁺Ly6G^–. Each dot represents an individual animal. Experiment was performed twice (mean ± SEM). (C) Cytometric bead arrays to quantify cytokine concentrations in serum taken from mice 2 days after wounding Each dot represents an individual animal. Experiment was performed twice (mean ± SEM).