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. 2020 May 5;11:835. doi: 10.3389/fmicb.2020.00835

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Copyright © 2020 Bai, Wang, Han and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD4⁺ T cell count, T-bet expression and mammalian target of rapamycin (mTOR) pathway activation differed according to degrees of sepsis. Splenocytes were obtained at 12 h after Candida injection. (A,B) CD4⁺ T cells were sorted by flow cytometry analysis. Protein levels of T-bet, phospho (p)-mTOR and p-P70S6 (C) in CD4⁺ T cells. Levels of T-bet, p-mTOR and p-P70S6 in CD4⁺ T cells were quantified by western blotting. The amount of each protein level was normalized by GAPDH. Mean ± SD, 10 mice per group, analyzed by one-way analysis of variance multiple comparisons. *P < 0.05, ***P < 0.001, ****P < 0.0001. WT, wild type; (Summarization of the flow cytometry scatter plots was provided in Supplementary Material).