FIGURE 4.

Expression of T-bet in different degrees of Candida sepsis is regulated by mTOR. (A–E) Protein levels of T-bet, phospho (p)-mTOR and p-P70S6 were quantified by western blotting from sorted CD4⁺ T cells in wild-type (WT), mTOR knockout (Lck-mTOR) and TSC1 knockout (Lck-TSC1) mice. The amount of each protein level was normalized by GAPDH. Mean ± SD, 10 mice per MSS group (“+CA” groups contained all infected mice of three MSS groups), analyzed by one-way analysis of variance multiple comparisons. *P < 0.05, **P < 0.01, ****P < 0.0001.