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. 2020 May 5;8:297. doi: 10.3389/fcell.2020.00297

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Copyright © 2020 Zhu, Wang, Shen, Zheng and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of SP1 expression phencopied the effect of miR-362-3p in RCC cells. (A) Western blot and qRT-PCR analysis showed reduction of SP1 protein and mRNA expression after siSP1 treatment. (B) The effect of siSP1 on the viability of RCC cells with CCK-8 assay. (C) The effect of siSP1 on the viability of RCC cells with Colony formation assay (Representative wells were presented). (D) The effect of siSP1 on the cell cycle progression with Flow cytometric analysis. (E) The effect of siSP1 on migration and invasion with Transwell assay (representative micrographs were presented), *P < 0.05.