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. 2020 May 5;8:406. doi: 10.3389/fbioe.2020.00406

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Copyright © 2020 Yan, Yin, Brochhausen, Pfeifer, Alt and Docheva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Collagen I protein production and gene expression profiling by Real Time PCR. (A) Representative collagen I immunofluorescent images of Y-TSPC and A-TSPC sheets at day 14. (B) Quantitative analysis of collagen I by ELISA. For (A,B) Y-TSPC n = 3; A-TSPC n = 6. Normalization to DNA. Quantitative PCR analysis for tendon-transcription factors (C), collagen genes (D), cross-linker genes (E), tendon-related ECM genes (F) other lineage genes (G). Gene expression was calculated as fold change to Y-TSPC group. GAPDH was used as reference gene in each group. Non-detectable (Ct over 38 genes were not included in the graphs. For (C–G) Y-TSPC n = 3; A-TSPC n = 3; ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001.