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. Author manuscript; available in PMC: 2020 May 12.
Published in final edited form as: Cell. 2019 Sep 12;179(1):132–146.e14. doi: 10.1016/j.cell.2019.08.025

Figure 2. TPPP is a Specific Marker for Golgi Outposts.

Figure 2.

(A) TPPP mRNA expression from RNA-seq of immunopanned mouse brain cells (Zhang et al., 2014). FPKM (frequency per kilobase million).

(B) Confocal micrograph of a rat DIV4 oligodendrocyte immunostained against TPPP, GM130, and tubulin.

(C) Venn diagram of colocalization relationship between TPPP and GM130 outside the cell body. 93% of TPPP+ puncta colocalize with GM130; 76% of GM130+ puncta colocalize with TPPP.

(D) Super-resolution (structured illumination) micrograph of a rat DIV4 oligodendrocyte immunostained against TPPP, GM130, and tubulin.

(E) Venn diagram of colocalization relationship between TPPP and EB1 outside the cell body. 35% of TPPP+ puncta colocalize with EB1; 41% of EB1+ puncta colocalize with TPPP.

(F) Confocal micrograph of rat DIV4 oligodendrocyte immunostained against tubulin, TPPP, and EB1.

(G) Kymographs from rat DIV1–2 oligodendrocytes expressing TPPP-GFP and mCherry-EB3.

(H) Comparison of actual rates of EB3-positive puncta arising from TPPP+ puncta versus probability based on chance. n = 9 cells, 3 biological replicates.

See also Figure S2.