Figure 6. TPPP KO Oligodendrocytes Have Shorter Myelin Sheaths.
(A) Schematic of techniques used to discern oligodendrocyte morphology.
(B) Max projections of confocal micrographs (100×) of DIV14 oligodendrocytes cultured from WT or TPPP KO mice on 3D microfibers. Cells were stained against GM130, tubulin, and MBP, then mounted on coverslips. Arrowheads point to Golgi outposts along the myelin sheath.
(C) Max projections (20×) of DIV14 oligodendrocytes cultured on 3D microfibers. Merged images show tubulin and MBP channels. Arrowheads point to cell bodies.
(D) Number of sheaths per cell. p = 0.57.
(E) Average sheath length per cell. ** p = 0.006.
(F) Total sheath length per cell. * p = 0.02.
(G) Microtubule sheath length per cell. ** p = 0.006. n = 3–4 biological replicates, 21–32 individual cells or cell clusters.
(H) Max projections of confocal micrographs of oligodendrocytes from P14 WT or TPPP KO cortex.
(I) Number of sheaths per cell. p = 0.77.
(J) Average sheath length per cell. ** p = 0.005.
(K) Total sheath length per cell. * p = 0.03.
(L) Combined radius of myelin sheath and axon. ** p = 0.001. n = 3 mice, 16–23 cells per genotype.