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. Author manuscript; available in PMC: 2021 Mar 3.
Published in final edited form as: Cell Metab. 2020 Feb 20;31(3):549–563.e7. doi: 10.1016/j.cmet.2020.01.011

Figure 5. The balance between mitochondrial and ribosomal protein translation is conserved in human cells.

Figure 5.

(A) Schematic of doxycycline (DOX) mediated inhibition of mitochondrial ribosomes with amoxicillin not interfering with mitochondria as control antibiotic.

(B) Polysome profiles of DMSO and amoxicillin (AMOX) controls and doxycycline (DOX)-treated K562 cells shows doxycycline-induced suppression of cytosolic translation.

(C) Western blot analyses of amoxicillin control and doxycycline-treated K562 cells shows doxycycline-induced expression of ATF4. Bars represent mean ± SD and significance was tested with 1way ANOVA with multiple comparisons, with α = 0.05