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Design of experiments for the optimization of chemical PCP lysis and extraction. Response surface of (A) DsRed and (B) TSP concentrations in PCP extracts depending on the concentrations of EDTA and SDS [% (m v– 1)] in the lysis buffer. Correlation of DsRed concentration after chemical lysis or mechanical extraction from PCPs for (C) apoplast-targeted (vector 000102) or (D) plastid-targeted products (vector 000104).
