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Lysis buffers tested for refined protein extraction from PCPs.
| Lysis buffer type | EDTA [mM] | SDS[% (m/v)] | Application | Modified based on |
| Harsh | 50.0 | 1.000 | Maximum lysis | Tsugama et al., 2011 |
| Mild | 1.0 | 0.009 | Preservation of tertiary structure | Bhuyan, 2010 |
| Opt 1 | 3.7 | 0.022 | Highly soluble and TSP | This study |
| Opt 2 | 7.3 | 0.002 | Highly soluble, low TSP | This study |
Opt, numerical optimization using DOE descriptive models.
