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. 2020 May 5;12:113. doi: 10.3389/fnagi.2020.00113

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Copyright © 2020 Christensen, Liu and Pike.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Measures of Alzheimer-related neuropathology in early- and late-MA female 3xTg-AD mice. Representative pictures of β-amyloid immunolabeling in the subiculum of the hippocampal formation from each treatment group in (A–D) early-MA and (G–J) late-MA female mice. β-Amyloid load was measured in the subiculum of (E) early-MA and (K) late-MA. Tau-immunolabeled cells were counted in the subiculum and CA1 hippocampal regions in (F) early-MA and (L) late-MA mice. Significance is p < 0.05. D = significant main effect of diet; H = significant main effect of hormone treatment; X = significant interaction between diet and hormone; d = significant post hoc effect between diets, but within hormone treatment; h = significant post hoc effect between hormone treatment, within diet.