TABLE 2.
Statistical analyses of data between age groups.
| Figure | Main effects, Interactions | Post hoc comparisons |
| 2A and 2F | Ftime × age × diet(4,104) = 55.8, p < 0.0001 Ftime × age × hormone(4,104) = 55.7, p < 0.0001 | 0 weeks: E-MA Con + Veh vs. L-MA Con + Veh, p < 0.001 E-MA HFD + Veh vs. L-MA HFD + Veh, p < 0.001 E-MA HFD + E2 vs. L-MA HFD + E2, p < 0.05 4 weeks: E-MA HFD + Veh vs. L-MA HFD + Veh, p < 0.0001 E-MA HFD + E2 vs. L-MA HFD + E2, p < 0.01 8 weeks: E-MA HFD + Veh vs. L-MA HFD + Veh, p < 0.0001 E-MA HFD + E2 vs. L-MA HFD + E2, p < 0.05 12 weeks: E-MA HFD + Veh vs. L-MA HFD + Veh, p < 0.01 16 weeks: E-MA HFD + Veh vs. L-MA HFD + Veh, p < 0.05 |
| 2B and 2G | Fage × diet(1,26) = 4.4, p < 0.05 | No significant differences |
| 2C and 2H | Fage × diet(1,26) = 24.6, p < 0.0001 Fage × hormone(1,27) = 29.7, p < 0.0001 | E-MA HFD + Veh vs. L-MA HFD + Veh, p < 0.001 E-MA HFD + E2 vs. L-MA HFD + E2, p < 0.05 |
| 2D and 2I | No significant main effects | |
| 2E and 2J | No significant main effects | |
| 3A and 3E | Ftime × age × diet(2,52) = 9.5, p < 0.001 Ftime × age × hormone(2,52) = 18.6, p < 0.0001 | No significant differences |
| 3B and 3F | Ftime × age × diet(4,96) = 174.5, p < 0.0001 Ftime × age × hormone(4,96) = 151.0, p < 0.0001 | No significant differences |
| 3C and 3G | No significant main effects | |
| 3D and 3H | Fage × diet(1,24) = 3.5, p = 0.08 | No significant differences |
| 4A and 4C | No significant main effects | |
| 4B and 4D | Fage × diet(1,26) = 28.5, p < 0.0001 | E-MA Control + Veh vs. L-MA Control + Veh, p < 0.001 E-MA HFD + Veh vs. L-MA HFD + Veh, p < 0.05 |
| 5E and 5K | Fage × diet(1,26) = 57.2, p < 0.0001 | E-MA Control + Veh vs. L-MA Control + Veh, p < 0.001 E-MA HFD + Veh vs. L-MA HFD + Veh, p < 0.0001 |
| 5F and 5L | Fage × diet(1,26) = 94.4, p < 0.0001 | E-MA Control + Veh vs. L-MA Control + Veh, p < 0.0001 E-MA HFD + Veh vs. L-MA HFD + Veh, p < 0.0001 |
| 6A and 6C | Fage × diet(1,25) = 28.0, p < 0.0001 Fage × hormone(1,26) = 69.6, p < 0.0001 | E-MA Control + Veh vs. L-MA Control + Veh, p < 0.05 E-MA HFD + Veh vs. L-MA HFD + Veh, p < 0.01 E-MA HFD + E2 vs. L-MA HFD + E2, p < 0.0001 |
| 6B and 6D | Fage × diet(1,25) = 44.6, p < 0.0001 Fage × hormone(1,26) = 141.3, p < 0.0001 | E-MA Control + Veh vs. L-MA Control + Veh, p < 0.0001 E-MA HFD + Veh vs. L-MA HFD + Veh, p < 0.01 E-MA HFD + E2 vs. L-MA HFD + E2, p < 0.0001 |
| 7A and 7D | Fdiet(1,51) = 17.3, p < 0.0001 Fdiet × hormone(1,51) = 5.4, p < 0.05 | No significant differences |
| 7B and 7E | Fdiet(1,51) = 11.1, p < 0.01 Fdiet × hormone(1,51) = 4.4, p < 0.05 | No significant differences |
| 7C and 7F | Fdiet(1,51) = 3.1, p = 0.09 Fage × diet(1,51) = 3.3, p = 0.08 | No significant differences |
E-MA, early middle age; L-MA, late middle age.