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. 2020 May 5;8:248. doi: 10.3389/fcell.2020.00248

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Copyright © 2020 Wang, Song, Zhang, Deng, Jiao, Qi, Lian and Yao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TLR4 effects on reactive oxygen species (ROS) production by TLR4 overexpression or RNAi. (A,B) Analysis of ROS by DCFH-DA in monocytes. ROS was detected by dichlorodihydrofluorescein diacetate, and a fluorescence microscope was used to count the positive cells, ×200. (C) ROS fluorescent positive cells; statistical analysis of not fewer than 100 cells. ^∗P < 0.05 vs. Wt group. (D) TLR4 RNAi effects on ROS production. ^∗P < 0.05 vs. NC-siRNA group.