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TLR4-induced p38 MAPK signaling and autophagy effect on intracellular oxidative stress. (A–C) Analysis of reactive oxygen species (ROS) in sheep. ROS was detected by the DCFH-DA probe and a fluorescence microscope was used to count the positive cells, ×400. (D) Level of NO. (E) Level of malondialdehyde. All data are presented as the mean ± SEM from three experiments. ∗P < 0.05 vs. LPS group; ∗∗P < 0.01 vs. LPS group.
