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Rhamnolipid precursor production was significantly reduced in the ΔntrBC double mutant when compared to the WT. Diameter of halo (mm) was measured following 120 h static incubation at room temperature (RT) on iron-limited salt medium. Data reported as mean ± standard error of the mean (SEM) from three independent experiments containing two biological replicates each (n = 6). **P < 0.05 according to Kruskal–Wallis nonparametric test followed by Dunn’s post hoc analysis.
