TABLE 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristicsa | References |
| Escherichia coli | ||
| XL-1 Blue | recA1 endA1 gyrA96 thi- 1 hsdR17 (rK- mK+) supE44 relA1 lac [F’ proAB laclq ZΔM15Tn10(Tcr)] | Stratagene |
| ST-18 | pro thi hsdR+ Tpr Smr; chromosome::RP4-2 Tc::Mu-Km::Tn7/λpir ΔhemA | Thoma and Schobert, 2009 |
| Pseudomonas aeruginosa | ||
| PA14 | WT P. aeruginosa UBCPP-PA14 | Rahme et al., 1995 |
| PA14 ΔntrB | PA14 ntrB chromosomal deletion | This study |
| PA14 ΔntrC | PA14 ntrC chromosomal deletion | This study |
| PA14 ΔntrBC | PA14 ntrBC chromosomal deletion | This study |
| LESB58 | WT P. aeruginosa Liverpool Epidemic Strain B58 | Cheng et al., 1996 |
| LESB58 ΔntrB | LESB58 ntrB chromosomal deletion | This study |
| LESB58 ΔntrC | LESB58 ntrC chromosomal deletion | This study |
| LESB58 ΔntrBC | LESB58 ntrBC chromosomal deletion | This study |
| Plasmids | ||
| pEX18Gm | Gene replacement vector, suicide plasmid carrying sacB, Gmr | Hoang et al., 1998 |
| pEX18Gm.ΔntrB | Cloned 0.94 kbp fusion fragment flanking ntrB, Gmr | This study |
| pEX18Gm.ΔntrC | Cloned 1.01 kbp fusion fragment flanking ntrC, Gmr | This study |
| pEX18Gm.ΔntrBC | Cloned 2.48 kbp fusion fragment flanking ntrBC, Gmr | This study |
| pBBR1MCS-5 | Broad host-range cloning vector, Gmr | Kovach et al., 1994 |
| pBBR-5.ntrB | Cloned 1.08 kbp ntrB gene, Gmr | This study |
| pBBR-5.ntrC | Cloned 1.44 kbp ntrC gene, Gmr | This study |
| pBBR-5.ntrBC | Cloned 2.51 kbp ntrBC gene, Gmr | This study |
aAntibiotics: gentamicin (Gm), tetracycline (Tc), trimethoprim (Tp), streptomycin (Sm).