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. 2020 May 5;11:730. doi: 10.3389/fimmu.2020.00730

FIGURE 5.

FIGURE 5

IL-17 deficiency downregulates inflammation in cartilage and intestine in MIA-injected IL-1Ra KO mice. IL-1Ra KO mice and IL-17-deficient IL-1Ra KO mice (DKO) were injected with 0.6 mg MIA in the right knee. At 4 weeks after the MIA injection, articular tissues and small intestine from each group of mice were isolated. (A–F) Sections of articular tissue from mice were immunostained for IL-1β (A), IL-6 (B), IL-17 (C), phosphorylated STAT3 (S727) (D), MCP-1, (E), and CCR2 (F). Representative histological features are shown (original magnification 400×). The results are quantified as means ± SDs for number of antibody-positive cells in three animals per group. (G) Sections of small intestine from mice were stained with hematoxylin and eosin. Representative histological features are shown (original magnification 100×). The graph shows the histological and inflammatory infiltrates score quantified. Data are means ± SDs. *P < 0.05, **P < 0.01, ***P < 0.001 vs. MIA-injected IL-1Ra KO mice group (2-tailed t-test).