Figure 6.

ASC adhesion on HT1080, T98G and Huh7. Analysis of FBS and SRGF binding potential (A), kinetics as well as adhesion stability (B) in dynamic flow conditions for HT1080, T98G and Huh7. In ASC adhesion strength test reported in (B), density of adherent FBS or SRGF ASC immediately after perfusion SM was expressed as 100% (first dot) and the fraction of adhering FBS or SRGF ASC along with the adhesion strength test was relatively expressed as percent. Perfusions were performed at 0.25 dyn/cm²; (C) representative images of fluorescently labeled FBS and SRGF ASC interacting with cancer cells after perfusions in the flow chamber. Perfusions were performed at 0.25 dyn/cm²; (D) expression of CD54 ICAM1 and CD106 VCAM1 in cancer cells analyzed by flow cytometry. SM, standard medium i.e., 10% FBS α-MEM medium added with 2% bovine serum albumin; *, P<0.01 versus SRGF ASC; ^§, P<0.01 versus HT1080 and T98G; c/mm², cells/mm².