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qRT-PCR and RNA-seq results on 4 circRNAs in clinical specimens. For qRT-PCR, the target gene expression was normalized to β-actin (ΔCt). For RNA-seq, the expression level was normalized to FPKM. Results are presented as mean ± SD (Normal-qRT-PCR, n=5; Case-qRT-PCR, n=6; Normal-FPKM, n=3; Case-FPKM, n=3). ***, P<0.001.
