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. 2020 May 5;11:602. doi: 10.3389/fphar.2020.00602

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Copyright © 2020 Wu, Wang, Wu, Xu, Zhang, Li, Zhang and Guo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TMP inhibits the protein expression of Col I, Col III, and a -SMA in HFs. (A) Morphologies of HFs were visualized after different concentrations of TMP (0, 1, 5, 10, 20, and 40 µM) under light microscope. (B) HFs treated with different concentrations of TMP were starved for 12–16 h in serum-free DMEM, harvested after 48 h, and subjected to western blot analysis. (C) Histogram of the results. All values were normalized to either the negative control. Error bars represented means ± SD of n = 4. *p < 0.05 and **p < 0.01 vs. control group.