Figure 3.

Neuronal nitric oxide synthase (nNOS) in corpora cavernosa. Representative images from rats with exposure only to room air for 12 weeks (N), smoke exposure for a full 12 weeks followed by immediate erectile function testing (S), smoke exposure for 12 weeks followed by 4 weeks of room air only exposure before erectile function testing (Q4W) and smoke exposure for 12 weeks followed by 8 weeks of room air only exposure before erectile function testing (Q8W). nNOS-positive fibers are strained brown with nNOS antibody. Positive staining for nNOS was less intense in the S group than in the N and Q8W groups. (Scale bars: left, 500 µm; right, 100 µm).