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. 2020 May 5;11:575. doi: 10.3389/fphar.2020.00575

Figure 6.

Figure 6

Britanin regulates the proteins levels of NF-κB p-65, p105/50, p-p-65, and regulates the mRNA levels of NF-κB p-65, p105/50 from cells treated with or without Britanin for 24 h. (A) Western blots of proteins extracted from MDA-MB-231 luc cells treated with or without Britanin with the indicated antibodies: NF-κB p-65, p105/50 and p-p-65, GAPDH was used as a control. The data of relative protein expression of NF-κB pathway presented in bar charts. (B) Western blots of proteins extracted from SUM-159 luc cells treated with or without Britanin with the indicated antibodies: NF-κB p-65, p105/50, p-p-65, GAPDH was used as a control. The data of relative protein expression of NF-κB pathway presented in bar charts. (C) Relative mRNA concentrations of NF-κB p-65, p105/50 in MDA-MB-231 luc cells. Data represent as the means ± S.D. of three independent experiments (n=3). (D) Relative mRNA concentrations of NF-κB p-65, p105/50 in SUM-159 luc cells. Data represent as the means ± S.D. of three independent experiments (n=3).