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Comparison of the R → O transitions in three different conditions. Time-resolved mode for N131V at pH 6.5 in dark blue and for WT at pH 6.0 in dark green; equilibrium mode for WT at pH 6.0 in red. All spectra normalized to 1 mM (kinetic—by amplitude of 1,965 cm-1 band, equilibrium—by 1661/1641 peaks difference).
