Table 1.
The MinION sequence results of ITS-PCR amplicons.
| ID # | Wildlife Species | Total reads obtaineda | Hit readsb (%) | Trypanosome species identification by MinION |
|---|---|---|---|---|
| 9 | Red lechwe | 556 | 289 (52.0%) | T. theileri |
| 10 | Hartebeest | 8830 | 7062 (80.0%) | T. godfreyi |
| 12 | Hartebeest | 467 | 278 (59.5%) | T. brucei |
| 14 | Hartebeest | 3138 | 2515 (80.1%) | T. godfreyi |
| 16 | Sable antelope | 65 | 54 (83.1%) | T. brucei |
| 20 | Sable antelope | 6319 | 3762 (59.5%) | T. theileri |
| 27 | Sable antelope | 93 | 55 (59.1%) | T. brucei |
| 34 | Buffalo | 302 | 93 (30.8%) | T. brucei |
| 39 | Buffalo | 547 | 439 (76.5%) | T. godfreyi |
| 44 | Buffalo | 2336 | 1956 (83.7%) | T. godfreyi |
| W1 | Warthog | 447 | 136/181 (30.4%/40.5%) | T. godfreyi/T. Simiae |
| W3 | Warthog | 571 | 412 (72.2%) | T. godfreyi |
| 7 | Impala | 482 | 454 (94.2%) | T. brucei |
| 19 | Impala | 24,213 | 11272 (46.6%) | T. godfreyi |
| 21 | Impala | 139 | 110 (79.1%) | T. brucei |
| 144 | Lion | 8221 | 7886 (46.6%) | T. congolense |
| 149 | Wild dog | 1047 | 822 (78.5%) | T. godfreyi |
a
The total read number obtained after de-indexing.
b
The obtained reads were blasted against BLASTn database, and the read number of the top hit are shown.