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. 2019 Dec 25;10(1):1–21. doi: 10.1016/j.jcmgh.2019.12.006

Figure 4.

Figure 4

Gls1 is increased in myofibroblasts and correlates with liver fibrosis during liver injury. Mice were treated with chow diet or CDAA-HFD for 12 or 22 weeks, or corn oil vehicle or CCl4 for 4–6 weeks. (A) Liver Gls1 and Slc1a5 mRNA was quantified by reverse-transcription polymerase chain reaction. Bars represent means ± SEM of n = 4–5 mice/group. *P < .05 vs chow diet or corn oil control. (B) Protein expression of Gls1 (both variants: KGA and GAC) measured by Western blot. (C) Sirius red staining and IHC staining of Gls1 in livers of mice treated with corn oil vehicle or CCl4, or chow diet or CDAA-HFD for 22 weeks. (D) αSMA and Gls1 co-immunofluorescent staining in livers from mice treated with CCl4. GAC, Glutaminase C; KGA, kidney-type glutaminase.