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. 2020 Apr 13;13(8):1836. doi: 10.3390/ma13081836

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Detection of calcium deposition via alizarin staining on undyed scaffold prototype coated with Saos-2 cells after 21 days: (a) left scaffold was seeded in RPMI-medium; (b) right scaffold was incubated with osteogenic differentiation medium; and (c) PCR results for osteogenic differentiation on Day 21. Saos-2 cells seeded on scaffolds were incubated with RPMI medium and osteogenic differentiation medium. Focus was on cDNA for osteogenic proteins: collagen 1 (Col1), alkalic phosphatase (ALP), and osteocalcin (BGLAP). GAPDH was used as internal standard. The 2^−(ΔΔCt) values are presented.