Table A1.
The 10 most common sequences in the Uad-, Ugu-, and Aad-modified ssDNA libraries.
| Clone Name | Sequence Ratio (a) | Random Region Sequence (b) | Clone Name | Sequence Ratio (a) | Random Region Sequence (b) |
|---|---|---|---|---|---|
| CRP-Uad1 | 27.5% | CGGttACAGAtGAtCAGGCtCAAACAGAtt | LDH-Uad1 | 8.4% | CACCCtCCAGACtAtAttCtAGGCAACCGA |
| CRP-Uad2 | 24.6% | AACtGGGtCGAACGCGGttACAGAtGAtCAG | LDH-Uad2 | 7.5% | tGtGtCGAtCAGAtGCGttACtAAAtCtCA |
| CRP-Uad3 | 17.4% | CCttCAAGAAttGCGGttACAGAtGAtCAG | LDH-Uad3 | 6.9% | AGGCCtCCCtCCtAtAttCtAGGCGACCGA |
| CRP-Uad4 | 11.9% | CCtCGtAtAAGACGGttACAGAtGAtCAGG | LDH-Uad4 | 6.2% | CCGGtAACACtAAtGtGAAtCtGACtAtAt |
| CRP-Uad5 | 10.7% | tGAtAAAAGCCCGGttACAGAtGAtCAGGG | LDH-Uad5 | 5.3% | CAtCCACtACGCCtAtAttCtAGGCGACCGA |
| CRP-Uad6 | 7.3% | ACCGGttACAGAtGAtCAGGGGCAAAGAAC | LDH-Uad6 | 4.5% | tGGGCtAtGGtACtAGACtGGCtCGGttGC |
| CRP-Uad7 | 0.13% | AtCGAAttGCtGGttACGGCAtCGAtCAGA | LDH-Uad7 | 2.7% | GtAACACtAAtCtCCCACCtCCCGtAtAtC |
| CRP-Uad8 | 0.04% | ACCGGttACAGAtGAtCAGGG | LDH-Uad8 | 2.5% | GtCGGttttCGCGtGGAGGtCCGtGAtCGG |
| CRP-Uad9 | 0.01% | AttGCtttAtGCCGGttACtGAtGAtCAGG | LDH-Uad9 | 2.4% | GGGtAttGGtCtCGACCACCGCtCGGAACC |
| CRP-Uad10 | 0.01% | AAtACGCtAtCCGtGtGCAGtCCGAAtGAt | LDH-Uad10 | 2.2% | tGtGtCGAtCAGAtGCGttACtAAtCtCA |
| CRP-Ugu1 | 27.9% | CAtGAAtGCGtGtGGttAtAGtAttGAACt | LDH-Ugu1 | 24.3% | CCtCCGCttGtGGAtACGAtGGACtAGtGG |
| CRP-Ugu2 | 14.3% | GtCtGAAAtCGCtttCCGGAtCGGACttAA | LDH-Ugu2 | 12.0% | ACCttAGACACGGtACttACCGACACtAAA |
| CRP-Ugu3 | 9.6% | GACGtCCCACGGtttGAtCAAACGtACAtA | LDH-Ugu3 | 8.9% | ttAGAtACttGGCtCtACttAttGACAAtC |
| CRP-Ugu4 | 6.5% | CtAGttCtGCCttAAtAtGGtCGGttAAGC | LDH-Ugu4 | 7.3% | CACtCCtGAttGCttAAGAtCttAGttCGA |
| CRP-Ugu5 | 5.9% | ACtCAAGttAtGCtGGACttCtttACAAAC | LDH-Ugu5 | 3.2% | tGtCttGGACACGGtACttACCtCACACtA |
| CRP-Ugu6 | 5.2% | GCAtACAACtCCCtAGtCAAACtGACAttA | LDH-Ugu6 | 2.5% | AtGtCCCttGGCtAtAGttCAAGtttAGAA |
| CRP-Ugu7 | 3.9% | GtCtGAAAtCAAtCtCGAttCGGAAttAtG | LDH-Ugu7 | 2.5% | GACACAtACttGGCtCtACAtAttGAGAGA |
| CRP-Ugu8 | 3.5% | AGAttCAGACCCCGAGGtAAtGCCGACtGt | LDH-Ugu8 | 1.6% | GGAGAtACttGGCtCtACttAttGACAAtC |
| CRP-Ugu9 | 3.6% | GAtGCGGtCtGAAAtCAAtCtAAAttCGCG | LDH-Ugu9 | 1.3% | ACCttCGACACGGtACttACCGACACtAAA |
| CRP-Ugu10 | 2.6% | GAACGGCtGCAtCCCCtAGtGGtGtAGGCA | LDH-Ugu10 | 1.2% | tAAGAtACttGGCtCtACttAttGACAAtC |
| CRP-Aad1 | 39.2% | aaCaTTGaGTGCCaTGCCCTTCGTaGaCa | LDH-Aad1 | 55.8% | CTGCTGGCTCGTGaGaCGGaTaTCaGGTCT |
| CRP-Aad2 | 19.5% | TTTaCCGaaTGCCaTGCCCGaGaGTaGaCa | LDH-Aad2 | 14.9% | aGaGGGaGaTCaTCTCTCTGGCGGaCaCaa |
| CRP-Aad3 | 10.0% | TCGaaCGCCaTGCCaCTGCCCGGTTaGaCa | LDH-Aad3 | 1.9% | CTGCTGGTTCGTGaGaCGGaTaTCaGGTCT |
| CRP-Aad4 | 5.1% | aCGTaGCaTaGTGTaaGGaGCGCCCaCTaT | LDH-Aad4 | 1.9% | CTaCTGGCTCGTGaGaCGGaTaTCaGGTCT |
| CRP-Aad5 | 4.2% | CaGCCGCCTGCCaTGCCCCCGCGGTaGaCa | LDH-Aad5 | 1.8% | CCGaCTCTaTGTGaGaGGGaGaTCaGCTCT |
| CRP-Aad6 | 3.5% | CaaaGTTaGGTGCTCCGTaCaCCaCTCTGC | LDH-Aad6 | 1.2% | CTGCTGGCTTGTGaGaCGGaTaTCaGGTCT |
| CRP-Aad7 | 3.3% | aaGaCaaTTGCCaTGCCCCCGTaGTaGaCa | LDH-Aad7 | 0.9% | CTGCTaGCTCGTGaGaCGGaTaTCaGGTCT |
| CRP-Aad8 | 2.9% | CaTGGCCaTGTCCCCCGGTaGaCaGCCGaT | LDH-Aad8 | 0.7% | aGaGGGaGaTCaTCTCTCTGGCGGaCaTaa |
| CRP-Aad9 | 2.3% | aaCCaaaTGCCaTGCCCCTCaGGTaGaCa | LDH-Aad9 | 0.7% | aGaCGGaTaGCaTGTCTCTGGCTGTGaTGT |
| CRP-Aad10 | 1.6% | TaTGTCGTaTGCCaTGCCCTGGaGTaGaCa | LDH-Aad10 | 0.7% | CTGCTGaCTCGTGaGaCGGaTaTCaGGTCT |
(a) Sequence ratio was defined as the ratio of the sequence from the total number of sequences that were generated by next-generation sequencing. (b) The letter “t” indicates Uad or Ugu, and the letter “a” indicates Aad.