Figure 7.

Immunohistochemical analysis of the effects of different treatments on B16.F10 murine melanoma microenvironment in vivo. (A) CD31 was used as a marker for proliferating endothelial cells; (B) F4/80 was used as a general marker for macrophages; (C) iNOS was used as a marker for M1 polarized macrophages. Positively stained cells appear in brown; Scale bar = 50 µm. The scores for immunoreaction intensities of tumor sections for each marker after different treatments were analyzed by using rank-based nonparametric Kruskall-Wallis test with Dunn’s test for multiple comparisons.