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. 2020 Apr 13;21(8):2688. doi: 10.3390/ijms21082688

Figure 5.

Figure 5

Chondrogenic differentiation of BMSCs in the static and dynamic cultural environment. Evaluation based on the different medium and culture system. (A,B) Immunohistochemistry staining with rabbit anti-human collagen II and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) on day 21, (100× Magnification; scale bar: 100 µm). (C,D) Proteoglycan expression and (E–H) RNA expression of BMSCs on GM in FDD, FDS, CMD, and CMS. All experiments based on BMSCs-GM were incubated in FD and CIM for 7, 14, and 21 days using static and dynamic culture condition in FD and CIM mediums. Figure 4. BMSC attachment and proliferation in the static and dynamic culture system. Evaluation based on the different medium and culture system. (A,B) A macroscopic view and proliferation of BMSCs-GM in FDS and FDD culture condition. (C,D) A macroscopic view and proliferation of BMSCs-GM in CMS and CMD culture condition. (E,F) A macroscopic view of BMSCs-GM in FDD, FDS, CMD, and CMS. All experiments based on BMSCs-GM incubated in FD and CIM for 7, 14, and 21 days using static and dynamic culture condition (FD-Static (FDS), CIM-Static (CMS), FD-Dynamic (FDD), and CIM-Dynamic (CMD)). The sample size (n) for each experiment was six (n = 6). (Figure 4E: 100× magnification; scale bar: 100 µm. Figure 4F: 3000× Magnification; scale bar: 40 µm). (FD-Static (FDS), CIM-Static (CMS), FD-Dynamic (FDD), CIM-Dynamic (CMD)). (p ≤ 0.001: FDS vs. FDD, CMS vs. CMD, FDS vs. CMS and FDD vs. CMD).