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. 2020 Apr 17;21(8):2823. doi: 10.3390/ijms21082823

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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EL4 TCR⁺ cells upregulate T helper cells (Th) type 17 genes in a manner similar to murine Th17 cells. Gene expression analysis of EL4 TCR⁺ cells (a) or Th17 polarized murine cells following an anti-CD3 stimulation (b). Il17a, Csf2, Rora, Rorc, Il21 and Il23 are markers specific for Th17. Ifng and Tbx 21 are markers of Th1, Il4 and Gata3 are markers of Th2, Il9, Foxp3 and Il10 are markers of Th9, Treg and Tr1, respectively. Data represent mean +/− standard deviation (SD). * p < 0.05, Student’s t-test.