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. 2020 Apr 12;21(8):2679. doi: 10.3390/ijms21082679

Figure 3.

Figure 3

The REC114-myc showed a synthetic defect in Rad51-foci formation with histone modification mutations. (A,B) Distribution of Rad51 foci numbers. The number of Rad51 foci in foci-positive nuclei (with more than 5 foci) in strains with the REC114-myc (A) and the MER2-myc and SPO11-FLAG (B) at indicated times were counted. At each time point, 42 spreads from three independent time courses (n = 126; 42 × 3) were analyzed. * p < 0.05; ** p < 0.01; *** p < 0.001. NS shows Not Significant. The p values and statistics for Rad51-foci numbers in foci-positive nuclei are shown in Table S2. The strains used are shown in Figure 1A. spp1 MER2-myc, ZYY1030; SPO11-FLAG ZYY1031; rtf1 SPO11-FLAG, ZYY1032. (C,D) Distributions of Rec114-myc- and Mer2-myc-foci numbers at 4, 5, and 6 h are shown. In REC114-myc (C), the number of myc foci in all spreads is shown. In MER2-myc (D), the number of myc foci in foci-positive nuclei (with more than 5 foci) is shown. At each time point, 42 spreads from three independent time courses (n = 126; 42 × 3) were analyzed. The p values and statistics are shown in Table S2.