The effect of IAA 0.5%, JA-Me 0.5% and their mixture on the formation of the secondary abscission zone depending on the place of treatment in the excised segments of the internode of B. calycinum. After the treatment, the internode segments were kept in the normal position (A–E) and the inverted position (F–J), and photographed ten days after treatments; (A,F) JA-Me applied on its own to the middle of the internode and lanolin to the top; (B,G) IAA applied together with JA-Me to the middle of internode and lanolin to the top; (C,H) IAA alone applied to the top of internode; (D,I) JA-Me and IAA applied together to the top; (E,J) JA-Me applied to the middle and IAA applied to the top of the segment. White arrows indicate the place of treatment, and white arrowheads indicate the place of the formation of the secondary abscission zone.