Figure 1.

Dextran sulfate sodium (DSS) treatment disrupts intestinal epithelial barrier without affecting cell viability. (A) DSS does not affect the viability or the morphological characteristics of Caco-2 at a concentration of 2% (w/v), for 24 h, as assessed by the lactate dehydrogenase (LDH) assay. Negative control contains only media (without cells); positive control cells were lysed prior to adding the LDH reaction mix (B) The integrity of Caco-2 cells monolayer treated with DSS was measured by trans-epithelial electrical resistance (TEER). Students t-test, * p < 0.05, SEM, n = 3.